im錢包下載安卓|馬血清
im錢包下載安卓|馬血清
- 作者: im錢包下載安卓
- 2024-03-08 02:00:51
Horse Serum for Cell Culture | Thermo Fisher Scientific - PH
Horse Serum for Cell Culture | Thermo Fisher Scientific - PH
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Home?Life Sciences?Cell Culture & Transfection?Mammalian Cell Culture?Other Animal Sera?Horse Serum for Cell CultureHorse Serum for Cell CultureSee Navigation?Other Animal SeraHorse Serum for Cell Culture
?Other Bovine Sera
?Rabbit Serum for Cell Culture
?
What is horse serum used for?
Gibco horse serum is donor-herd collected in New Zealand and can be used as an alternative to fetal bovine serum.Horse serum has been cited for use in several specific cell culture applications:Support of in vitro antibody response to SRBC in place of FBS [1]Production of C2 heterokaryons [2]Isolation and culture of primary neural cells [3]Promotion of myogenic differentiation [4]Supplement in mycoplasma broths and agars [5]In addition to horse serum as a lower cost alternative to FBS, Thermo Fisher Scientific also offers normal horse serum that can be used as a blocker solution in immunoassays.Gibco horse serum is available in both heat-inactivated and non-heat-inactivated formats.
Horse serum vs FBS
Horse serum can be used as a viable FBS alternative in some cell culture applications. One key horse serum benefit compared to FBS is its lower cost. However, horse serum composition does differ from FBS composition, with horse serum tending to exhibit lower growth factor and higher immunoglobulin levels than FBS [6]. Therefore, due to differences in FBS and horse serum, your specific experiment and needs should be carefully considered when deciding whether to use horse serum.
Horse serum collection, manufacturing, and testing
Collected from donor herds in New Zealand, each lot of horse serum is tested for its ability to support the growth of Sp2/O-Ag14 (murine myeloma) cells in control medium containing the test lot of serum. Results are compared to those obtained using control growth medium with a previously characterized reference serum. In addition, Gibco horse serum is EIA (equine infectious anemia) tested.
Order horse serum
16050130, 16050122, 16050114, 26050070, 26050088
Publications
Levy EM. (1980) The ability of horse serum to support an in vitro antibody response. J Immunol Methods 36(2):181–183.?Production of Heterokaryons. Stanford University Protocols.Patel MR, Weaver AM. (2021) Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF-β signaling. Cell Rep 34(10):108829.?Sosa P, Alcalde-Estévez E, Asenjo-Bueno A, et al. (2021) Aging-related hyperphosphatemia impairs myogenic differentiation and enhances fibrosis in skeletal muscle. J Cachexia Sarcopenia Muscle 12(5):1266–1279.?Ligasová A, Vydr?alová M, Buriánová R, et al. (2019) Koberna K. A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 8(12):1510.?Subbiahanadar Chelladurai K, Selvan Christyraj JD, Rajagopalan K, et al. (2021) Alternative to FBS in animal cell culture - An overview and future perspective. Heliyon 7(8):e07686. ??
Resources
Fetal Bovine Serum Basics Learn the basics of FBS for cell culture, including information on the FBS uses, components, and the market dynamics driving this industry.Cell Culture BasicsLearn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.Gibco iMatch Sera Lot Matching Tool?Provide your previous Gibco FBS lot number or answer a few questions to find your ideal serum match.Related productsCell culture mediaCell culture plasticsCell culture reagentsCell culture instruments and equipmentTransfection products
Support
Find your regional sales representativeConnect with a regional FBS sales representative.Cell Culture & Transfection Support CenterFind technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.Need technical support?Contact our expert team for technical and application support of laboratory products.For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.Stylesheet for Classic Wide Template adjustments
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特級胎牛血清,馬血清 - 武漢普諾賽生命科技有限公司 - 您身邊的細胞專家【官方網(wǎng)站】
特級胎牛血清,馬血清 - 武漢普諾賽生命科技有限公司 - 您身邊的細胞專家【官方網(wǎng)站】
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普諾賽?血清來自南美純正血源，涵蓋特級胎牛血清和馬血清，多種規(guī)格可選，滿足不同的實驗需求。血清生產(chǎn)過程符合cGMP標準,通過多項質量測試，低內毒素,無細菌、支原體、噬菌體、病毒等污染，無外源添加因子，不含激素等。 經(jīng)過近600種細胞系、500多種原代細胞，十萬次以上細胞培養(yǎng)驗證，只為您放心使用。 普諾賽優(yōu)質純正的南美胎牛血清，促進細胞增殖, 可用于培養(yǎng)腫瘤細胞、原代細胞以及部分干細胞。 歷經(jīng)多年發(fā)展，普諾賽公司一直在持續(xù)穩(wěn)定的為廣大藥物研發(fā)、疫苗研發(fā)企業(yè)及醫(yī)院、高校等科研機構工作者供應高品質血清，歡迎咨詢選購。
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普諾賽?血清培養(yǎng)細胞生長支撐實驗
* 注明: 通過更精細化的生長曲線繪制，使用特定對血清較敏感的細胞系以及部分原代細胞進行測試，用CCK-8檢測，繪制一周的生長曲線?？梢愿黠@的看出，我們的血清質量在同類型產(chǎn)品中名列前茅。
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普諾賽?文獻數(shù)量已超過4000余篇，其中已有大量客戶使用我們的血清產(chǎn)品開展實驗，并取得優(yōu)秀成果，在Advanced Science、Redox Biology、Environmental Pollution等高分雜志上多次發(fā)表文章。
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Cuttlebone-Derived Organic Matrix: A Facile Periosteum Substitute for Bone Regeneration
IF: 19.000
期刊：Advanced Functional Materials
DOI：10.1002/adfm.202214095
引用產(chǎn)品： 164210 164210-50
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Wnt3a-Loaded Extracellular Vesicles Promote Alveolar Epithelial Regeneration after Lung Injury
IF: 15.100
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DOI：10.1002/advs.202206606
引用產(chǎn)品： 164210-50 CL-0016
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血清產(chǎn)品系列
由于血清的特性，為了解決血清也不適宜反復凍融的問題，方便客戶使用，我們的血清產(chǎn)品有50ml、100ml、500ml以及50ml*10等不同規(guī)格包裝。可滿足不同的實驗需求
血清FAQs
普諾賽特級胎牛血清適合培養(yǎng)哪些細胞？
普諾賽特級胎牛血清具有非常卓越的促細胞生長能力，適合培養(yǎng)腫瘤細胞，原代細胞，部分干細胞和嬌貴細胞。
血清如何保存, 可以放多久？
貯藏溫度≤-15℃，可長時間保存； 2～8℃存放時，請勿超過一個月。
血清溶解以后，出現(xiàn)沉淀怎么辦?
若您欲去除這些絮狀沉淀物，可以將血清分裝至無菌離心管內，以400-600g離心5min，上清液即可加入培養(yǎng)基內一起培養(yǎng)。 我們不建議您以過濾的方法去除這些絮狀沉淀物， 一方面它可能會阻塞您的過濾膜；另一方面，過濾血清這種行為可能會導致血清中部分營養(yǎng)成分的流失。
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細胞培養(yǎng)中“低調的高手”——馬血清 - 知乎
細胞培養(yǎng)中“低調的高手”——馬血清 - 知乎切換模式寫文章登錄/注冊細胞培養(yǎng)中“低調的高手”——馬血清靈兒細胞培養(yǎng)中的馬血清說起細胞培養(yǎng)用的血清，大多數(shù)人都會想到胎牛血清、新生牛血清，對于馬血清卻知之甚少。今天就給大家介紹一下血清中“低調的高手”——馬血清。一、馬血清作用細胞培養(yǎng)級的馬血清，經(jīng)過多次過濾，去除了許多大分子蛋白，但保留了血清中促細胞生長的活性因子，表現(xiàn)出與胎牛血清和牛血清的類似的特性，因而能用于細胞培養(yǎng)。馬血清可以刺激成纖維細胞分化，以及細胞外基質 (ECM) 、細胞因子的合成。另外培養(yǎng)神經(jīng)細胞和心肌細胞、誘導生成樹突狀細胞等實驗均可用馬血清替代胎牛血清。因此，馬血清通常適用于那些研究有絲分裂后，細胞關于生長因子和激素等問題的實驗?；蛟S，我們可以這樣說，從現(xiàn)有研究來看，馬血清更適合用來研究特定種類細胞的分化。實際實驗應用方面：馬血清已被用作腦皮質神經(jīng)元-膠質細胞培養(yǎng)的一種必添加物；大鼠的 PC12 細胞，在培養(yǎng)過程中，也需要在基礎培養(yǎng)基中添加馬血清作為補充劑。1. 馬血清可用于細胞培養(yǎng)和干細胞的誘導分化，例如：（1）RPMI-1640培養(yǎng)液加馬血清培養(yǎng)臍帶血單個核細胞。（參考文獻：李春莉, 王建偉, 姜蓉, et al. 人參總皂苷誘導紅系血細胞增殖的信號轉導[J]. 中國組織工程研究, 2009(23):4568-4572.）（2）原代培養(yǎng)神經(jīng)細胞。有人用含10%馬血清的DMEM，分離培養(yǎng)BALB/c小鼠原代腦神經(jīng)細胞。發(fā)現(xiàn)和添加2%神經(jīng)細胞生長劑B27的Neurobasal培養(yǎng)基相比，含10%馬血清的DMEM培養(yǎng)基更節(jié)省成本，方便廉價。（參考文獻：李丹, 呂耀中, 王微,等. 兩種培養(yǎng)基分離培養(yǎng)BALB/c小鼠原代神經(jīng)細胞方法的比較[C]// 中國北方實驗動物科技年會. 遼寧省實驗動物學會, 2015.）（3）誘導脂肪干細胞向成肌細胞分化（文獻來源：劉杰,傅強.馬血清在脂肪干細胞成肌誘導中的作用[J].中國組織工程研究, 15(14)）（4）培養(yǎng)骨髓基質細胞。與胎牛血清相比，馬血清對小鼠骨髓基質細胞的促增殖作用更好。（文獻來源：王改琴. 當歸多糖對小鼠骨髓基質細胞黏附分子表達及其黏附功能的影響[D]. 學位論文.重慶醫(yī)科大學, 2008.）（5）添加20%馬血清的L-DMEM培養(yǎng)液可誘導人骨髓間充質干細胞分化為脂肪細胞。（文獻來源：趙文靜，陳亞潔，劉薇.人骨髓間充質干細胞分離培養(yǎng)及生物學特性研究.中國現(xiàn)代醫(yī)學雜志.2006.16(18):2744-2747）2. 馬血清添加到微生物培養(yǎng)基中，可用于培養(yǎng)微生物，如：（1）馬血清添加到培養(yǎng)基中用于支原體的培養(yǎng)。在《獸藥典》中，含馬血清的支原體培養(yǎng)基被用于獸用疫苗的支原體檢查。用KM2培養(yǎng)基(含馬血清)還可培養(yǎng)豬肺炎支原體。（文獻來源：胡茂志 焦新安,等.豬肺炎霉形體單克隆抗體的制備與鑒定.《中國獸醫(yī)科技》 2004年12期）（2）馬血清加入到固體和液體培養(yǎng)基中，可用于培養(yǎng)幽門螺桿菌。并且，和羊血清和牛血清比較，添加馬血清的培養(yǎng)基更易于分裝和保存。（文獻來源：劉琳娜, 丁士剛, 張靜,等. 幾種培養(yǎng)幽門螺桿菌方法的比較[J]. 胃腸病學, 2012, 017(004):240-241.）3. 馬血清及激素可共同誘導建立家兔股骨頭缺血壞死的模型。（文獻來源：洪嵩, 張?zhí)旌? 非創(chuàng)傷性股骨頭壞死模型的血液流變學及脂質代謝[C]// 2009第一屆貴州骨科論壇.）二、注意事項與胎牛血清一樣，馬血清在使用過程中也應注意幾個原則：1、避免反復凍融；2、三步法解凍，及時分裝；3、配制好的完全培養(yǎng)基在4℃儲存不要超過7天。還有一個大家比較關心的問題，馬血清是否需要滅活？我們都知道，胎牛血清由于特殊的采血時間（5-8月胎齡），牛胚胎體內還沒有形成完整的補體系統(tǒng)，因而不需要采用熱滅活的手段對補體進行滅活。而馬血清，在沒有特殊說明的情況下，一般采自成年馬匹。作為脊椎動物，成年馬也擁有完整的補體系統(tǒng)。因而，如果所進行的實驗中，涉及補體相關內容，建議大家在使用前對馬血清進行熱滅活，也可以直接購買商品化的滅活馬血清。一些比較成功的實驗案例中，所用到的馬血清，也是經(jīng)過熱滅活的。相關實驗請參考：https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5111218/Ausbian馬血清為細胞培養(yǎng)級。它來自健康馬血液，為封閉系統(tǒng)內無菌采血過濾而成，為淡黃色澄清液體，超低內毒素，無溶血，無異物，無細菌、真菌、支原體、噬菌體等。產(chǎn)品描述1、每個批次均具有完整的檢測報告，其中含有下列檢測：1）理化檢查包括外觀、pH值、滲透壓、血紅蛋白、總蛋白、內毒素、蛋白顆粒度等2）無菌檢查含細菌真菌檢查、支原體檢查、噬菌體檢查，確保不含這些外源因子。3）病毒檢查牛病毒性腹瀉病毒（BVDV）為陰性。2、極低內毒素。3、提供試用，養(yǎng)細胞更安心。發(fā)布于 2022-05-15 21:58細胞死亡細胞細胞學?贊同 3??添加評論?分享?喜歡?收藏?申請
Horse Serum, heat inactivated, New Zealand origin
Horse Serum, heat inactivated, New Zealand origin
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HomeShop All ProductsCell Lines and Blood ProductsSeraHorse and Equine SeraHorse Serum, heat inactivated, New Zealand originGibco?Horse Serum, heat inactivated, New Zealand originCatalog number: 26050070Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceGibco?Horse Serum, heat inactivated, New Zealand originCatalog number: 26050070Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceCatalog Number26050070also known as 26050-070Unit Size100 mLPrice (USD)Contact Us ?Save to listCatalog Number26050088Unit Size500 mLPrice (USD)Contact Us ?Save to listCatalog NumberUnit SizePrice (USD)26050070also known as 26050-070100 mLContact Us ?26050088500 mLContact Us ?Save to listProduct OverviewFiguresVideosRecommendationsRecommendationsDocumentsFAQCitations & ReferencesAdditional InformationRecommendationsGibco? sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirementsSera Category: SpecialtyOrigin: New Zealand.EIA tested (negative) serum from a donor herd.SpecificationsSpeciesHorse/Equine, HorseCountry of OriginNew ZealandFormLiquidProduct TypeHorse SerumQuantity100 mLSerum TreatmentHeat-inactivatedSterilitySterileProduct LineGibco?Shipping ConditionFrozenContents & StorageStorage conditions: ≤-10°CShipping conditions: FrozenFiguresDocuments & DownloadsCertificatesSearch by lot number or partial lot numberSearchRequest a CertificateFrequently asked questions (FAQs)Citations & ReferencesSearch citations by name, author, journal title or abstract textSearch
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馬血清給特別滴細胞特別滴“愛” - 知乎
馬血清給特別滴細胞特別滴“愛” - 知乎切換模式寫文章登錄/注冊馬血清給特別滴細胞特別滴“愛”absin愛必信-absin，中國人自己的生命科學百寶箱Σ眾所周知，胎牛血清是細胞培養(yǎng)界的“香餑餑”，備受胞胞們的“寵愛”，其實還有一個“香煎餅”，大家可知道？對一些“挑食”的細胞，馬血清可謂是散發(fā)著一股“煎餅的清香”。圖1 馬血清優(yōu)級（abs989）一、馬血清的第一大功能是“滋養(yǎng)”一些特別的細胞，如免疫細胞類、成纖維細胞類、上皮細胞類、神經(jīng)細胞類等。需要添加馬血清的常見細胞類型如下：1）NK（人外周血自然殺傷細胞）和NK-92（人惡性非霍奇金淋巴瘤患者的自然殺傷細胞）營養(yǎng)體系：74% α-MEM基礎培養(yǎng)基（abs9506）＋ 0.2mM Inositol（abs816057）＋ 0.1mM β-mercaptoethanol（abs9592）＋ 0.02mM Folic Acid（abs812845）＋ 100-200U/mL recombinant IL-2（abs04045）+ 12.5 %馬血清優(yōu)級（abs989）+ 12.5% 胎牛血清優(yōu)級（abs972）+ 1% 雙抗（abs9244）2）EL-4（小鼠胸腺淋巴瘤細胞）營養(yǎng)體系：89% DMEM高糖基礎培養(yǎng)基（abs9483）+ 10% 馬血清優(yōu)級（abs989）+ 1% 雙抗（abs9244）3）L929（小鼠成纖維細胞）和CRFK（貓腎細胞）營養(yǎng)體系：89% MEM基礎培養(yǎng)基（abs9503）+ 10% 馬血清優(yōu)級（abs989）+ 1% 雙抗（abs9244）4）MCF 10A（人正常乳腺上皮細胞）營養(yǎng)體系：94% DMEM/F12基礎培養(yǎng)基（abs9561）+ 5% 馬血清（abs989）+ 1% 雙抗（abs9244）+ 20ng/ml EGF（abs04176）+ 0.5ug/ml 氫化可的松Hydrocortisone（abs814512）+ 100ng/ml 霍亂霉素choleratoxin+10ug/ml 胰島素insulin（abs42225219）5）PC-12（Undifferentiated）（大鼠腎上腺嗜鉻細胞瘤細胞（未分化））營養(yǎng)體系：79% RPMI1640基礎培養(yǎng)基（abs9484）+ 15% 馬血清（abs989）+ 5% 胎牛血清優(yōu)級（abs972）+ 1% 雙抗（abs9244）二、馬血清第二大功能是誘導分化作用，有文獻報道【1】馬血清在 5-氮雜胞苷誘導脂肪干細胞成肌細胞實驗中發(fā)揮重要作用。該研究如下：將第1代長滿100 mm培養(yǎng)皿的脂肪干細胞傳代至3個6孔板，實驗組培養(yǎng)基中加入體積分數(shù)為 5% 馬血清 +10 μmol/L 5-氮雜胞苷；對照組培養(yǎng)基中單純添加 10 μmol/L 的 5-氮雜胞苷；空白組為單純低糖 DMEM 培養(yǎng)基，其余培養(yǎng)、傳代及鑒定所需的條件相同。每日光鏡觀察記錄形態(tài)，在培養(yǎng)的第 7、14、28、35 天行免疫熒光和流式細胞儀檢測肌蛋白表達差異。該文獻的結果與結論是免疫熒光檢測成肌特異性胞質蛋白表達以及流式細胞檢測相應蛋白表達率提示，加入馬血清后的實驗組脂肪干細胞的成肌速度和誘導成肌所需時間明顯比單純加入 5-氮雜胞苷的對照組更有優(yōu)勢。實驗初步表明馬血清在促進脂肪干細胞誘導成肌方面起著縮短誘導成肌所需時間的作用。同時，馬血清在誘導 C2C12（小鼠成肌細胞）分化為多核肌管方面也發(fā)揮重大作用。C2C12 細胞是由 D·Yaffe 和 O·Saxel 建立的小鼠成肌細胞株的亞克??；該細胞分化很快，形成可伸縮的肌管并生成特征性的肌蛋白。將狀態(tài)良好的骨骼肌細胞以 0.9×105 接種在96孔板上（每孔100ul），置于培養(yǎng)箱中培養(yǎng) 24h 后，待細胞融合到 70-80% 后，更換成的含有 2% 馬血清的完全培養(yǎng)基中，進行誘導分化。（如圖2所示）圖3 C2C12（小鼠成肌細胞）向多核肌管方向分化另外，也有文獻報道【2】傳代培養(yǎng)人骨髓間充質干細胞（P3）在 20% 馬血清的 L-DMEM 培養(yǎng)液中可分化為脂肪細胞。此外， 10% 馬血清可代替 FBS 用于支持綿羊紅細胞的體外抗體應答； 血清 IgG 制備，白蛋白的提取及生物活性研究；免疫組化（IHC）、酶聯(lián)免疫（ELISA）等免疫實驗的封閉劑。參考文獻：【1】劉杰,傅強.馬血清在脂肪干細胞成肌誘導中的作用[J].中國組織工程研究, 15(14)【2】趙文靜,陳亞潔,劉薇.人骨髓間充質干細胞分離培養(yǎng)及生物學特性研究.中國現(xiàn)代醫(yī)學雜志.2006.16(18):2744-2747本期講解就到這里了，如有細胞培養(yǎng)問題，歡迎微信公眾號后臺留言~注：圖源網(wǎng)絡和客戶，僅供參考學習用表 細胞培養(yǎng)類產(chǎn)品推薦（點擊查看更多產(chǎn)品信息）貨號品名規(guī)格abs981胎牛血清（特級）500mlabs972胎牛血清（優(yōu)級）500mlabs974胎牛血清(標準級）500mlabs9483高糖DMEM培養(yǎng)基500mlabs9484RPMI-1640培養(yǎng)基500mlabs9505MEM培養(yǎng)基500ml發(fā)布于 2023-05-05 10:59?IP 屬地上海細胞培養(yǎng)NK小鼠細胞?贊同 1??添加評論?分享?喜歡?收藏?申請
Fetal Bovine Serum (FBS) | Thermo Fisher Scientific - PH
Fetal Bovine Serum (FBS) | Thermo Fisher Scientific - PH
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Home?Life Sciences?Cell Culture & Transfection?Mammalian Cell Culture?Fetal Bovine Serum (FBS)Fetal Bovine Serum (FBS) for Cell CultureContact us for the specialist support ?Fetal bovine serum (FBS) offers essential nutrients and growth factors for the maintenance and growth of cultured cells. Cell-based applications are an integral part of basic research, biochemical studies, vaccine development, and drug discovery to name a few. FBS is the most used serum in cell-based applications as it provides the most robust culture system for the widest range of cell types.Since 1962, Gibco FBS products have been a global market leader. Through Thermo Fisher Scientific’s unwavering commitment to quality, scientists using Gibco FBS have been provided with consistent reliability, service, value, and innovation. Discover the performance differences that Gibco Fetal Bovine Serum can deliver for your research and production.
Performance based on science, not geography
Specifications, not origin, help drive consistent performance. We commissioned an independent research group to survey more than 500 researchers around the globe. This is what we learned:Only 7% of FBS users believe that country of origin is the most important indicator of FBS quality.Almost 80% said they would purchase from categories that were clearly delineated by quality indicators (i.e., endotoxin, hemoglobin, growth performance, virus testing, etc.) that matter to their research.Based on these findings, we transformed our Gibco FBS portfolio into categories focused on quality indicators rather than origin. Now you can easily select the best sera for your needs based on how they perform, not where they came from. This also enables us to minimize supply disruption since there is less reliance on origin.The benefits of our performance-based categories Sustainable supply—more supply through origin flexibility assists in minimizing supply disruption Performance consistency—more supply means more customer choice and more lots with the specifications they desire Product continuity—remove the hassle of updating protocols, which can be based on specifications, the true performance driver, and not part numbers
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We provide a simplified three-tiered offering—Gibco Value FBS, Premium FBS, and Premium Plus FBS—where each category is clearly delineated by relevant performance markers and testing levels to help ensure you can confidently select the right serum for your research.
Value FBS
Sera for standard research applications with up to 30 quality specification testsOrder Value FBSOrder HI Value FBS
Premium FBS
Our most popular FBS product; high quality sera and exceptional value with up to 60 qualities specification testsOrder Premium FBSOrder HI Premium FBS
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Our highest quality FBS for use with the most sensitive cells; up to 70 quality specification tests, including our lowest levels of endotoxin and hemoglobin release specificationsOrder Premium Plus FBSAU originOrder Premium Plus FBSUS originOrder Premium Plus FBSNZ originOrder HI Premium Plus FBSAU origin
Specialty FBS
Sera qualified for specialty research; specific assays, including stem cell research, immunoassays, antibodies, and othersExplore Specialty FBS
Explore our comprehensive FBS quality control release criteria for Value FBS, Premium FBS, and Premium Plus FBS
Quality profileValue FBSPremium FBSPremium Plus FBSEndotoxin?≤20 EU/mL≤10 EU/mL≤5 EU/mLHaemoglobin/hemoglobin?≤25 mg/dL≤25 mg/dL≤20 mg/dLSterility: bacterial and fungal testing???Osmolality???pH???Mycoplasma: supplemental testing (H-stain)???Mycoplasma???Country of origin???Oritain? testing (origin confirmation)–??Biochemical and hormonal profileBiochemical: Alkaline phosphatase, ALT, AST, bicarbonate, bilirubin (total), BUN, BUN/creatinine ratio, calcium, chloride, cholesterol, creatinine, GGPT, glucose, HDL, iron, iron saturation, LDH, LDL, phosphorous (inorganic), potassium, sodium, TIBC, triglycerides (TG), uric acid–??Hormonal: Estradiol, insulin, progesterone, testosterone, thyroxine (T4)–??Virus testingSerology: BVDV serum neutralization titer (Ab)–??Serology: Anti-BVDV antibody test (EMA lots only)–??Virus (9CFR): Bluetongue Virus, Bovine Adenovirus, Bovine Parvovirus, Bovine Viral Diarrhoea Virus, Bovine Respiratory Syncytial Virus (BRSV), Rabies Virus, Reo Virus Cytopathogenic Agents - Including Bovine Herpesvirus 1 (BHV-1/IBR) Haemadsorbing Agents - Including Bovine Parainfluenza Virus 3 (PI-3)???Virus (EMA): Bluetongue Virus, Bovine Adenovirus, Bovine Parvovirus, Bovine Viral Diarrhoea Virus, Bovine Respiratory Syncytial Virus (BRSV), Rabies Virus, Reo Virus Cytopathogenic Agents- Including Bovine Herpesvirus 1 (BHV-1/IBR) Haemadsorbing Agents- Including Bovine Parainfluenza Virus 3 (PI-3)–??USDA safety testing: Bluetongue virus (Mexican and Australian origin)? (Mexican only)??? (Australian only)?USDA safety testing: Akabane virus (Australian only)–?? (Australian only)Protein electrophoresisIdentification:Electrophoretic profile???Identification: Bovine gamma globulin (≤500 mg/L)????Protein: Albumin, alpha globulin, beta globulin, total protein???Performance testingRelative growth promotion (RGP), relative cloning efficiency (RCE), relative plating efficiency (RPE)???DocumentationCertificate of Suitability (TSE CEP)–??Important to note that our FBS is sourced from BSE-negligible countriesGibco Fetal Bovine Serum cross-reference toolThe Gibco Fetal Bovine Serum cross reference tool can be used to find a direct replacement for other serum suppliers.?Find your replacement
Discover heat-inactivated and One Shot fetal bovine serum
Heat-inactivated FBS
Choose heat-inactivated FBS for immunological applications requiring the inactivation of complement proteins.Explore heat-inactivated FBS
One Shot FBS
Choose One Shot FBS in convenient 50 mL bottles to end aliquoting and help minimize contamination risk.Explore One Shot FBS
Video: What should you consider when selecting FBS for cell culture?
Learn what scientists consider some of the most important factors in selecting fetal bovine serum for mammalian cell culture.Fetal bovine serum (FBS) is a byproduct of harvesting cattle for the meatpacking industry–it offers essential growth factors for the maintenance and growth of cultured cells. FBS is used as a supplement to basal growth medium in cell culture applications.Explore FBS productsContact us for the specialist supportComing soon Gibco iMATCH Sera Lot Matching Tool
Find the most consistent, highest performing serum lot available for your research—all without having to test. Be the first to know when this exciting selection tool becomes available in your country. Get notified
Customer story: using Gibco FBS for cell culture
Learn how Fanny at Institut Curie in Paris uses FBS to culture cells, supporting studies of cell mechanisms and behaviors.
Why Gibco FBS is recommended by most scientists worldwide
Since its launch over 60 years ago, Gibco FBS has been a part of historical scientific breakthroughs, including cell line development, cloning, and gene editing. Gibco sera are the most cited in global scientific journals and are used and trusted by many top pharma companies. Furthermore, Gibco FBS is origin guaranteed through fingerprinting technology and is ISIA traceability certified.Learn more about FBS fingerprinting and ISIA traceability certification
Extraordinary measures in Gibco FBS manufacturing
Extraordinary measures are taken during the manufacturing and production of Gibco FBS, with careful consideration taken in each step of the journey from farm to bench.Explore the incredible manufacturing journey of Gibco FBS
Other animal sera
Did you know there are lower-cost alternatives to fetal bovine serum? These products include bovine serum, horse serum, newborn calf serum, goat serum, rabbit serum, porcine serum, and chicken serum.Explore other animal sera
Sera for bioprocessing
Gibco sera for bioprocessing can support your cell culture needs as you scale up production. As one of the largest serum suppliers from BSE-negligible risk regions, Gibco serum can help meet biopharmaceutical manufacturing requirements.Learn more about sera for bioprocessing
Featured resources and product help for fetal bovine serum
Download the Gibco guide booklet
Learn more about how we’ve become a world leader for sera, media, and reagents.?Download PDF
Understand the basics of fetal bovine serum
Discover the important factors to consider in FBS selection and learn how scientists are utilizing the benefits of FBS in their research.Learn more
Contact an FBS representative
We have dedicated regional sales representatives to assist you in all things FBS. Connect directly with a FBS representative today!Contact us
Get your answers using the FBS FAQ database
Have questions about using FBS in your cell culture experiments? Find the answers you need in this extensive FAQ database.Access FBS FAQs?Fewer resources | Less wasteWhen minimizing waste in your lab, every little bit helps. The 1 L Gibco bottle uses 38% less source material (32% less for the 0.5 L bottle) than comparable media bottles on the market today. This helps reduce the consumption of non-renewable resources, helping shrink your footprint.?Download the Green Fact SheetLearn more about sustainable solutions
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Fetal Bovine Serum Basics Learn the basics of FBS for cell culture, including information on the FBS uses, components, and the market dynamics driving this industry.Cell Culture BasicsLearn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.Gibco iMatch Sera Lot Matching Tool?Provide your previous Gibco FBS lot number or answer a few questions to find your ideal serum match.Related productsCell culture mediaCell culture plasticsCell culture reagentsCell culture instruments and equipmentTransfection products
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Find your regional sales representativeConnect with a regional FBS sales representative.Cell Culture & Transfection Support CenterFind technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.Need technical support?Contact our expert team for technical and application support of laboratory products.For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.Stylesheet for Classic Wide Template adjustments×Extraordinary MeasuresThank you for joining us on our extraordinary FBS journey. Check your email for a special code that can be used to download our one-of-a-kind, fun, science-based emojis.Continue the FBS journey with our extraordinary Gibco sera products.Close×NEW?Gibco Premium Plus FBS—our most defined serumWe developed this product to answer the demand for a more stringent quality serum, which is ideal for vaccine, therapeutic, diagnostic, and other demanding research applications.Learn more or get a free sample today
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HyClone 供體馬血清_細胞培養(yǎng)工藝_思拓凡官網(wǎng) | Cytiva
HyClone 供體馬血清_細胞培養(yǎng)工藝_思拓凡官網(wǎng) | Cytiva
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HyClone 供體馬血清，美國血源
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根據(jù) 9 CFR 113.53 進行了病毒檢測
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給這些動物喂以豐富的食物，確保它們攝取適量營養(yǎng)，并在放血前禁食，以降低其脂質濃度。馬匹在專門為無菌采集而配備的密閉設施中放血。每匹馬均常規(guī)進行 Coggins 馬傳染性貧血試驗。供體馬血清經(jīng)過三重連續(xù) 100 nm (0.1 μm) 額定孔徑過濾器過濾。在分裝前，各批血清將使用真混合技術進行混合，以確保各瓶之間的均勻性和一致性。其他可用的處理方法包括熱滅活和輻照。還可進行其他測試，如符合 EMEA 的測試。
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淺談「馬血清」在細胞生物學中的作用 - 知乎
淺談「馬血清」在細胞生物學中的作用 - 知乎切換模式寫文章登錄/注冊淺談「馬血清」在細胞生物學中的作用愛生物的小蟲子深耕在生物圈內的科研狗細胞培養(yǎng)用Ausbian馬血清馬血清經(jīng)過多次過濾，祛除了許多大分子蛋白，但保留了血清中促細胞生長的活性因子，因而能用于細胞培養(yǎng)。相關科學研究1、馬血清可用于細胞培養(yǎng)或誘導樹突狀細胞，且細胞生長、增殖狀況良好。有研究人員分別用胎牛血清和馬血清培養(yǎng)馬單核細胞來產(chǎn)生樹突狀細胞（eqMoDC），結果發(fā)現(xiàn)，馬血清馬血清生成的eqMoDC與FBS生成的eqMoDC沒有區(qū)別。然而，與馬血清補充培養(yǎng)基一起孵育的eqMoDC在從單核細胞分化過程中表現(xiàn)出更具特征性的樹突狀細胞形態(tài)。在用馬血清培養(yǎng)的eqMoDC中也觀察到細胞活力的顯著增加。此外，發(fā)現(xiàn)在馬血清存在下產(chǎn)生的eqMoDC在功能性T淋巴細胞啟動能力方面優(yōu)越，并且引發(fā)的非特異性增殖顯著減少。參考文獻：李春莉, 王建偉, 姜蓉, et al. 人參總皂苷誘導紅系血細胞增殖的信號轉導[J]. 中國組織工程研究, 2009(23):4568-4572.Ziegler A, Everett H, Hamza E, Garbani M, Gerber V, Marti E, Steinbach F. Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum. BMC Vet Res. 2016 Nov 15;12(1):254. doi: 10.1186/s12917-016-0880-8. PMID: 27846835; PMCID: PMC5111218.2、馬血清可用于原代培養(yǎng)神經(jīng)細胞。有人用含10%馬血清的DMEM，分離培養(yǎng)BALB/c小鼠原代腦神經(jīng)細胞。發(fā)現(xiàn)和添加2%神經(jīng)細胞生長劑B27的Neurobasal培養(yǎng)基相比，含10%馬血清的DMEM培養(yǎng)基更節(jié)省成本，方便廉價。參考文獻李丹, 呂耀中, 王微,等. 兩種培養(yǎng)基分離培養(yǎng)BALB/c小鼠原代神經(jīng)細胞方法的比較[C]// 中國北方實驗動物科技年會. 遼寧省實驗動物學會, 2015.3、特定脂肪酸產(chǎn)物濃度的馬血清，對造血細胞有支持作用研究表明，磷脂酶A2特定脂肪酸產(chǎn)物的濃度馬血清，支持多能小鼠造血祖細胞FDCP-Mix細胞自我更新的能力。參考文獻Ditz T, Schnapka-Hille L, Noack N, Dorow J, Ceglarek U, Niederwieser D, Schiller J, Fuchs B, Cross M. Phospholipase A2 products predict the hematopoietic support capacity of horse serum. Differentiation. 2019 Jan-Feb;105:27-32. doi: 10.1016/j.diff.2018.12.002. Epub 2018 Dec 6. PMID: 30554008.4、馬血清可增強豬單性生殖胚胎植入前，胚層的發(fā)育。有研究通過對比不同血清和血清樣物質，對豬單性生殖胚胎植入前發(fā)育的影響，尋找適合其發(fā)育的培養(yǎng)基。對單性生殖胚胎進行豬濾泡液（PFF）、胎牛血清（FBS）、馬血清（HS）或豬血清白蛋白（PSA）處理，培養(yǎng)2天后。通過囊胚形成和孵化，發(fā)現(xiàn)馬血清是增強胚層發(fā)育的最有效蛋白質來源。接下來，使用三種不同濃度的HS（10%，20%和30%）來確定改善豬單性生殖胚胎發(fā)育所需的適宜HS濃度。所有HS濃度均增加了囊胚細胞數(shù)量，降低了囊胚凋亡細胞的發(fā)生率，其中20%效果更顯著。參考文獻Hwang IS, Kim SW, Byun SJ, Kim KW, Chung HK, Park JJ, Gobianand K, Park JK, Yoo JG. The effect of horse serum on in vitro development of porcine parthenogenetic embryos. Reprod Biol. 2012 Mar;12(1):25-39. doi: 10.1016/s1642-431x(12)60075-8. PMID: 22472938.5、馬血清添加到微生物培養(yǎng)基中，可用于培養(yǎng)微生物，如：（1）馬血清添加到培養(yǎng)基中用于支原體的培養(yǎng)。在《獸藥典》中，含馬血清的支原體培養(yǎng)基被用于獸用疫苗的支原體檢查。用KM2培養(yǎng)基(含馬血清)還可培養(yǎng)豬肺炎支原體。（2）馬血清加入到固體和液體培養(yǎng)基中，可用于培養(yǎng)幽門螺桿菌。并且，和羊血清和牛血清比較，添加馬血清的培養(yǎng)基更易于分裝和保存。參考文獻胡茂志 焦新安,等.豬肺炎霉形體單克隆抗體的制備與鑒定.《中國獸醫(yī)科技》 2004年12期劉琳娜, 丁士剛, 張靜,等. 幾種培養(yǎng)幽門螺桿菌方法的比較[J]. 胃腸病學, 2012, 017(004):240-241.Ausbian馬血清產(chǎn)品說明Ausbian馬血清為細胞培養(yǎng)級。它來自健康馬血液，為封閉系統(tǒng)內無菌采血過濾而成，為淡黃色澄清液體，超低內毒素，無溶血，無異物，無細菌、真菌、支原體、噬菌體等。產(chǎn)品描述中國國內屠場采集，當?shù)卣F醫(yī)管理局檢查合格。每個批次均具有完整的檢測報告，其中含有下列檢測檢測報告（1）理化檢查包括外觀、pH值、滲透壓、血紅蛋白、總蛋白、內毒素、蛋白顆粒度等。（2）無菌檢查含細菌真菌檢查、支原體檢查、噬菌體檢查，確保不含這些外源因子。（3）病毒檢查牛病毒性腹瀉病毒（BVDV）等病毒均為陰性。極低內毒素，含量≤5EU/ml，使細胞更健康。可提供試用，養(yǎng)細胞更放心。發(fā)布于 2022-09-25 22:59細胞培養(yǎng)?贊同??添加評論?分享?喜歡?收藏?申請